: Determine The cycle at which the second derivative of fluorescence with regard to cycle amount is maximal (Rasmussen, 2001). Neighborhood fitting into a parabola could be used to determine the peak place with sub‐cycle precision. 10 Commercially accessible RNA extraction kits such as the Qiagen RNeasy® Mini Plant package https://www.facebook.com/NewTechFind/posts/pfbid02RiZtF2CYQRNXKgPH6trquBJHCScc84k1QCRwYeyZWmLUNxJHv6cRNfFN7v8cEgq9l