The transcription factor Gli3 is acting mainly as a transcriptional repressor in the Sonic hedgehog signal transduction pathway. Gli3 contains a repressor domain in its N-terminus from residue G106 to E236. In this study we have characterized the intracellular structure of the Gli3 repressor domain using a combined bioinformatics and experimental approach. https://www.diegojavierfares.com/mega-pick-SA439-EF75-5-x-7-Embroidery-Machine-Hoop-mega-pick/